Immunofluorescent Studies of Viral Antigens in Mouse Leukemias

Viral and Cellular Surface Antigens of Murine Leukemias and Myelomas

IMMUNOELECTRON MICROSCOPY (IEM) OF MOUSE CELLS WHICH WERE PRODUCTIVELY INFECTED WITH MURINE LEUKEMIA VIRUS (MULV) YIELDED THE FOLLOWING CONCLUSIONS: See PDF for Structure With two exceptions, the alloantigens H-2, theta, Ly-A, and Ly-B were not present on complete or incomplete virions produced by cells bearing these antigens.The exceptions were H-2(k) (but not H-2(b)) and theta both appeared o...

Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys.

A method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to Lassa, Marburg, and Ebola viruses. The technique uses mixtures of specifically infected Vero cells placed on Teflon-templated microscopy slides. It was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly h...

Immunofluorescent Studies in Human Leukemia1

High-speed (80,000 X g) pellets of plasma from leukemic patients and from normal individuals were used to prepare antisera in rabbits. The leukemic plasma pellets contained virus-like particles as determined by electron microscopy, whereas similar particles were not observed in the normal pellets. Globulin fractions from six antisera to normal pellets (NPA) and six antisera to leukemic pellets ...

Studies on Avian Viral Infections in Iran

Characterization of antigens on acute lymphoblastic leukemias.

Detectable differences in surface membrane characteristics of normal lymphocyte populations have facilitated the classification of leukemic cells according to cell origin and Stage of differentiation. Recent reports show that distinct leukemic cell populations do not only express markers found on normal cell populations. Using specific antisera new antigenic determinants on cells of common acut...